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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Blood Banking | 15–20% | - Blood products and components - Blood group systems and immunology - Transfusion practice and testing |
| Topic 2: Urinalysis and Other Body Fluids | 5–10% | - Microscopic analysis and disease correlation - Physical and chemical testing |
| Topic 3: Immunology | 5–10% | - Principles of immunology - Serologic procedures and immune diseases |
| Topic 4: Microbiology | 15–20% | - Virology and parasitology testing - Bacteriology and mycology procedures |
| Topic 5: Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
| Topic 6: Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Topic 7: Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. FFP that has been thawed at 30 - 37oC and maintained at 1 - 6oC must be transfused within 24 hours. In contrast, "Thawed Plasma" can be used for up to 5 days as a replacement therapy for patients requiring stable clotting factors. Keep in mind that these are two different component types and you are asked about FFP.
Blood Bank
FFP that has been thawed at 30 - 37oC and maintained at 1 - 6oC must be transfused within ___________ after it has been thawed.
A) 8 hours
B) 12 hours
C) 5 days
D) 10 days
E) 24 hours
2. The increase in inflammatory cytokines and the abnormal lipids and lipid levels often present in metabolic syndrome increase the risk of cardiovascular disease. Adiponectin is decreased and increases in angiotensinogen lead to hypertension.
Chemistry
Which of the following statements is true regarding cardiovascular disease and adipose tissue? Please select all correct answers
A) Inflammatory cytokines, synthesized and released by adipose tissue, contribute to the development of cardiovascular disease
B) Obesity in metabolic syndrome contributes to the development of cardiovascular disease by increased synthesis and secretion of adiponectin
C) Increased synthesis and secretion of PAI-1 in metabolic syndrome contributes to cardiovascular disease by increasing blood pressure
D) A state of abnormal lipids and lipid levels found in metabolic syndrome contribute to the development of cardiovascular disease
3. Interferon gamma serves various purposes in immunologic response, including the promotion of natural killer cell activity as well as decreased viral replication by using enzymes to decrease protein replication, both viral or host, inside of the cell.
The lymphokine most important for increased natural killer cell activity and decreased viral replication in cells is:
A) Interferon gamma
B) Interleukin 3
C) Interleukin 5
D) Interleukin 4
4. CAMP is the correct answer because the case is typical of Streptococcus agalactiae(Group B strep). The main presumptive identification test is CAMP. Bile solubility is positive for Streptococcus pneumonia. Coagulase would be positive for Staphylococcus aureus. PYR is positive for Streptococcus pyogenes (Group A strep) which is ruled out by the resistance to bacitracin.
Microbiology
Spinal fluid cultures on a 3-day-old infant revealed beta-hemolytic, Gram-positive cocci occurring in pairs and chains. The organism was catalase-negative and bile esculin-negative. Resistance to 0.04 U disk of bacitracin was noted. The other test necessary to identify this organism is a positive test by:
A) Bile solubility
B) CAMP
C) Coagulase
D) PYR
5. Medical Technology is the profession concerned with performing laboratory analyses
A) False
B) True
Solutions:
| Question # 1 Answer: E | Question # 2 Answer: A,D | Question # 3 Answer: A | Question # 4 Answer: B | Question # 5 Answer: B |


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